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human setd2 sirna  (Qiagen)


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    Qiagen human setd2 sirna
    U2OS cells were transfected with the indicated siRNAs (15 pmol total), incubated for 2 days, and Bmal1-dLuc rhythms were measured for 5–6 days following dexamethasone synchronization. Knocked-down cells were assayed in either MEM high glucose (yellow lines) or MEM low glucose (blue lines) medium. Averaged luciferase traces are shown for each glucose concentration without detrending or further data manipulation ( N = 6 biological/technical replicates for AllStars negative controls; N = 4 biological/technical replicates for other siRNA knockdowns per nutrient concentration; standard deviation error bars). siRNA knockdown efficiency was measured using RT-qPCR relative to nontransfected control samples . Approximately 90% knockdown was achieved for CPSF6 , and approximately 70% knockdown for <t>SETD2</t> under these experimental conditions ( N = 2 biological replicate samples each with N = 3 technical replicate RT-qPCR reactions). Period lengths were calculated and averaged for all replicates ( A ). siRNA knocked-down cells were assayed in either DMEM 30 mM glucose (yellow lines) or DMEM 10 mM glucose (blue lines) medium. One representative luciferase trace is shown ( N = 7–10 biological/technical replicates per nutrient concentration). Period lengths were calculated and averaged for all replicates and plotted as boxplots ( B ). Average period lengths were as follows: 23.2 ± 1.1 hours (low glucose control) and 23.5 ± 0.6 hours (high glucose control); 23.6 ± 1.1 hours (low glucose SETD2 knockdown); and 25.2 ± 1.8 hours (high glucose SETD2 knockdown) (* p < 0.05, Student t test). The raw data underlying parts ( A ) and ( B ) can be found in .
    Human Setd2 Sirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+setd2+sirna/human+setd2+sirna/pmc09848017-365-36-39
    Average 90 stars, based on 1 article reviews
    human setd2 sirna - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability"

    Article Title: Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability

    Journal: PLOS Biology

    doi: 10.1371/journal.pbio.3001961

    U2OS cells were transfected with the indicated siRNAs (15 pmol total), incubated for 2 days, and Bmal1-dLuc rhythms were measured for 5–6 days following dexamethasone synchronization. Knocked-down cells were assayed in either MEM high glucose (yellow lines) or MEM low glucose (blue lines) medium. Averaged luciferase traces are shown for each glucose concentration without detrending or further data manipulation ( N = 6 biological/technical replicates for AllStars negative controls; N = 4 biological/technical replicates for other siRNA knockdowns per nutrient concentration; standard deviation error bars). siRNA knockdown efficiency was measured using RT-qPCR relative to nontransfected control samples . Approximately 90% knockdown was achieved for CPSF6 , and approximately 70% knockdown for SETD2 under these experimental conditions ( N = 2 biological replicate samples each with N = 3 technical replicate RT-qPCR reactions). Period lengths were calculated and averaged for all replicates ( A ). siRNA knocked-down cells were assayed in either DMEM 30 mM glucose (yellow lines) or DMEM 10 mM glucose (blue lines) medium. One representative luciferase trace is shown ( N = 7–10 biological/technical replicates per nutrient concentration). Period lengths were calculated and averaged for all replicates and plotted as boxplots ( B ). Average period lengths were as follows: 23.2 ± 1.1 hours (low glucose control) and 23.5 ± 0.6 hours (high glucose control); 23.6 ± 1.1 hours (low glucose SETD2 knockdown); and 25.2 ± 1.8 hours (high glucose SETD2 knockdown) (* p < 0.05, Student t test). The raw data underlying parts ( A ) and ( B ) can be found in .
    Figure Legend Snippet: U2OS cells were transfected with the indicated siRNAs (15 pmol total), incubated for 2 days, and Bmal1-dLuc rhythms were measured for 5–6 days following dexamethasone synchronization. Knocked-down cells were assayed in either MEM high glucose (yellow lines) or MEM low glucose (blue lines) medium. Averaged luciferase traces are shown for each glucose concentration without detrending or further data manipulation ( N = 6 biological/technical replicates for AllStars negative controls; N = 4 biological/technical replicates for other siRNA knockdowns per nutrient concentration; standard deviation error bars). siRNA knockdown efficiency was measured using RT-qPCR relative to nontransfected control samples . Approximately 90% knockdown was achieved for CPSF6 , and approximately 70% knockdown for SETD2 under these experimental conditions ( N = 2 biological replicate samples each with N = 3 technical replicate RT-qPCR reactions). Period lengths were calculated and averaged for all replicates ( A ). siRNA knocked-down cells were assayed in either DMEM 30 mM glucose (yellow lines) or DMEM 10 mM glucose (blue lines) medium. One representative luciferase trace is shown ( N = 7–10 biological/technical replicates per nutrient concentration). Period lengths were calculated and averaged for all replicates and plotted as boxplots ( B ). Average period lengths were as follows: 23.2 ± 1.1 hours (low glucose control) and 23.5 ± 0.6 hours (high glucose control); 23.6 ± 1.1 hours (low glucose SETD2 knockdown); and 25.2 ± 1.8 hours (high glucose SETD2 knockdown) (* p < 0.05, Student t test). The raw data underlying parts ( A ) and ( B ) can be found in .

    Techniques Used: Transfection, Incubation, Luciferase, Concentration Assay, Standard Deviation, Quantitative RT-PCR

    Related Articles

    Formulation:

    Article Title: Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability
    Article Snippet: Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) [ ] using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates. .. Although the Opti-MEM medium formulation is not publicly available, one study reported the Opti-MEM glucose concentration as 2.5 g/L or 13.88 mM [ ]. siRNAs were obtained from Qiagen: AllStars Negative Control siRNA (Qiagen # 1027280); human SETD2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS29072, 4× siRNAs used at 3.75 pmol each); human CPSF6 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS11052, 4× siRNAs used at 3.75 pmol each); human CRY2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS1408, 4× siRNAs used at 3.75 pmol each) [ ]. ..

    Article Title: A role for gene expression and mRNA stability in nutritional compensation of the circadian clock
    Article Snippet: Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) ( ) using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates. .. Although the Opti-MEM medium formulation is not publicly available, one study reported the Opti-MEM glucose concentration as 2.5 g/L or 13.88 mM ( ). siRNAs were obtained from Qiagen: AllStars Negative Control siRNA (Qiagen # 1027280); human SETD2 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS29072, 4x siRNAs used at 3.75 pmol each); human CPSF6 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS11052, 4x siRNAs used at 3.75 pmol each); human CRY2 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS1408, 4x siRNAs used at 3.75 pmol each) ( ). ..

    Concentration Assay:

    Article Title: Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability
    Article Snippet: Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) [ ] using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates. .. Although the Opti-MEM medium formulation is not publicly available, one study reported the Opti-MEM glucose concentration as 2.5 g/L or 13.88 mM [ ]. siRNAs were obtained from Qiagen: AllStars Negative Control siRNA (Qiagen # 1027280); human SETD2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS29072, 4× siRNAs used at 3.75 pmol each); human CPSF6 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS11052, 4× siRNAs used at 3.75 pmol each); human CRY2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS1408, 4× siRNAs used at 3.75 pmol each) [ ]. ..

    Article Title: A role for gene expression and mRNA stability in nutritional compensation of the circadian clock
    Article Snippet: Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) ( ) using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates. .. Although the Opti-MEM medium formulation is not publicly available, one study reported the Opti-MEM glucose concentration as 2.5 g/L or 13.88 mM ( ). siRNAs were obtained from Qiagen: AllStars Negative Control siRNA (Qiagen # 1027280); human SETD2 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS29072, 4x siRNAs used at 3.75 pmol each); human CPSF6 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS11052, 4x siRNAs used at 3.75 pmol each); human CRY2 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS1408, 4x siRNAs used at 3.75 pmol each) ( ). ..

    Negative Control:

    Article Title: Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability
    Article Snippet: Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) [ ] using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates. .. Although the Opti-MEM medium formulation is not publicly available, one study reported the Opti-MEM glucose concentration as 2.5 g/L or 13.88 mM [ ]. siRNAs were obtained from Qiagen: AllStars Negative Control siRNA (Qiagen # 1027280); human SETD2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS29072, 4× siRNAs used at 3.75 pmol each); human CPSF6 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS11052, 4× siRNAs used at 3.75 pmol each); human CRY2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS1408, 4× siRNAs used at 3.75 pmol each) [ ]. ..

    Article Title: A role for gene expression and mRNA stability in nutritional compensation of the circadian clock
    Article Snippet: Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) ( ) using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates. .. Although the Opti-MEM medium formulation is not publicly available, one study reported the Opti-MEM glucose concentration as 2.5 g/L or 13.88 mM ( ). siRNAs were obtained from Qiagen: AllStars Negative Control siRNA (Qiagen # 1027280); human SETD2 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS29072, 4x siRNAs used at 3.75 pmol each); human CPSF6 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS11052, 4x siRNAs used at 3.75 pmol each); human CRY2 siRNA (Qiagen # 1027416, FlexTube GeneSolution GS1408, 4x siRNAs used at 3.75 pmol each) ( ). ..



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    U2OS cells were transfected with the indicated siRNAs (15 pmol total), incubated for 2 days, and Bmal1-dLuc rhythms were measured for 5–6 days following dexamethasone synchronization. Knocked-down cells were assayed in either MEM high glucose (yellow lines) or MEM low glucose (blue lines) medium. Averaged luciferase traces are shown for each glucose concentration without detrending or further data manipulation ( N = 6 biological/technical replicates for AllStars negative controls; N = 4 biological/technical replicates for other siRNA knockdowns per nutrient concentration; standard deviation error bars). siRNA knockdown efficiency was measured using RT-qPCR relative to nontransfected control samples . Approximately 90% knockdown was achieved for CPSF6 , and approximately 70% knockdown for <t>SETD2</t> under these experimental conditions ( N = 2 biological replicate samples each with N = 3 technical replicate RT-qPCR reactions). Period lengths were calculated and averaged for all replicates ( A ). siRNA knocked-down cells were assayed in either DMEM 30 mM glucose (yellow lines) or DMEM 10 mM glucose (blue lines) medium. One representative luciferase trace is shown ( N = 7–10 biological/technical replicates per nutrient concentration). Period lengths were calculated and averaged for all replicates and plotted as boxplots ( B ). Average period lengths were as follows: 23.2 ± 1.1 hours (low glucose control) and 23.5 ± 0.6 hours (high glucose control); 23.6 ± 1.1 hours (low glucose SETD2 knockdown); and 25.2 ± 1.8 hours (high glucose SETD2 knockdown) (* p < 0.05, Student t test). The raw data underlying parts ( A ) and ( B ) can be found in .
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    U2OS cells were transfected with the indicated siRNAs (15 pmol total), incubated for 2 days, and Bmal1-dLuc rhythms were measured for 5–6 days following dexamethasone synchronization. Knocked-down cells were assayed in either MEM high glucose (yellow lines) or MEM low glucose (blue lines) medium. Averaged luciferase traces are shown for each glucose concentration without detrending or further data manipulation ( N = 6 biological/technical replicates for AllStars negative controls; N = 4 biological/technical replicates for other siRNA knockdowns per nutrient concentration; standard deviation error bars). siRNA knockdown efficiency was measured using RT-qPCR relative to nontransfected control samples . Approximately 90% knockdown was achieved for CPSF6 , and approximately 70% knockdown for SETD2 under these experimental conditions ( N = 2 biological replicate samples each with N = 3 technical replicate RT-qPCR reactions). Period lengths were calculated and averaged for all replicates ( A ). siRNA knocked-down cells were assayed in either DMEM 30 mM glucose (yellow lines) or DMEM 10 mM glucose (blue lines) medium. One representative luciferase trace is shown ( N = 7–10 biological/technical replicates per nutrient concentration). Period lengths were calculated and averaged for all replicates and plotted as boxplots ( B ). Average period lengths were as follows: 23.2 ± 1.1 hours (low glucose control) and 23.5 ± 0.6 hours (high glucose control); 23.6 ± 1.1 hours (low glucose SETD2 knockdown); and 25.2 ± 1.8 hours (high glucose SETD2 knockdown) (* p < 0.05, Student t test). The raw data underlying parts ( A ) and ( B ) can be found in .

    Journal: PLOS Biology

    Article Title: Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability

    doi: 10.1371/journal.pbio.3001961

    Figure Lengend Snippet: U2OS cells were transfected with the indicated siRNAs (15 pmol total), incubated for 2 days, and Bmal1-dLuc rhythms were measured for 5–6 days following dexamethasone synchronization. Knocked-down cells were assayed in either MEM high glucose (yellow lines) or MEM low glucose (blue lines) medium. Averaged luciferase traces are shown for each glucose concentration without detrending or further data manipulation ( N = 6 biological/technical replicates for AllStars negative controls; N = 4 biological/technical replicates for other siRNA knockdowns per nutrient concentration; standard deviation error bars). siRNA knockdown efficiency was measured using RT-qPCR relative to nontransfected control samples . Approximately 90% knockdown was achieved for CPSF6 , and approximately 70% knockdown for SETD2 under these experimental conditions ( N = 2 biological replicate samples each with N = 3 technical replicate RT-qPCR reactions). Period lengths were calculated and averaged for all replicates ( A ). siRNA knocked-down cells were assayed in either DMEM 30 mM glucose (yellow lines) or DMEM 10 mM glucose (blue lines) medium. One representative luciferase trace is shown ( N = 7–10 biological/technical replicates per nutrient concentration). Period lengths were calculated and averaged for all replicates and plotted as boxplots ( B ). Average period lengths were as follows: 23.2 ± 1.1 hours (low glucose control) and 23.5 ± 0.6 hours (high glucose control); 23.6 ± 1.1 hours (low glucose SETD2 knockdown); and 25.2 ± 1.8 hours (high glucose SETD2 knockdown) (* p < 0.05, Student t test). The raw data underlying parts ( A ) and ( B ) can be found in .

    Article Snippet: Although the Opti-MEM medium formulation is not publicly available, one study reported the Opti-MEM glucose concentration as 2.5 g/L or 13.88 mM [ ]. siRNAs were obtained from Qiagen: AllStars Negative Control siRNA (Qiagen # 1027280); human SETD2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS29072, 4× siRNAs used at 3.75 pmol each); human CPSF6 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS11052, 4× siRNAs used at 3.75 pmol each); human CRY2 siRNA (Qiagen # 1027416, FlexiTube GeneSolution GS1408, 4× siRNAs used at 3.75 pmol each) [ ].

    Techniques: Transfection, Incubation, Luciferase, Concentration Assay, Standard Deviation, Quantitative RT-PCR